Table 11. Results obtained from the examination of UGV-2, S5-like and TSMV-2 specific S-segments by RT-PCR and UGV-1, UHV-1 NP and UHV-1 NP-C specific IgY and IgM antibodies by ELISA—Animals without BIBD.
| RT-PCR | UGV-1 | UHV-1 NP | UHV-1 NP-C | ||||||
|---|---|---|---|---|---|---|---|---|---|
| IgY | IgM | IgY and IgM | IgY | IgM | IgY and IgM | IgY | IgM | IgY and IgM | |
| Positive/ Negative 36/36 (100%) |
Pos 24/36 (66.67%) | Pos 16/36 (44.44%) |
Pos 10/36 (27.78%) |
Pos 17/36 (47.22%) | Pos 9/36 (25%) | Pos 8/36 (22.22%) |
Pos 19/36 (52.78%) | Pos 17/36 (47.22%) | Pos 16/36 (44.44%) |
| Neg 12/36 (33.33%) | Neg 20/36 (55.56%) |
Neg 6/36 (16.67%) |
Neg 19/36 (52.78%) | Neg 27/36 (75%) | Neg 18/36 (50%) |
Neg 17/36 (47.22%) | Neg 19/36 (52.78%) | Neg 16/36 (44.44%) |
|
| Positive 32/36 (88.89%) | Pos 21/32 (65.63%) |
Pos 14/32 (43.75%) |
Pos 8/32 (25.00%) |
Pos 16/32 (50%) |
Pos 9/32 (28.13%) |
Pos 8/32 (25.00%) |
Pos 17/32 (53.13%) |
Pos 16/32 (50%) |
Pos 15/32 (46.88%) |
| Neg 11/32 (34.38%) |
Neg 18/32 (56.25%) |
Neg 5/32 (15.63%) |
Neg 16/32 (50%) |
Neg 23/32 (71.88%) | Neg 15/32 (46.88%) |
Neg 15/32 (46.88%) |
Neg 16/32 (50%) |
Neg 14/32 (43.75%) |
|
| 3 Segments 7/32 (21.88%) | Pos 5/7 (71.43%) |
Pos 3/7 (42.86%) |
Pos 1/7 (14.29%) |
Pos 4/7 (57.14%) |
Pos 2/7 (28.57%) |
Pos 1/7 (14.29%) |
Pos 3/7 (42.86%) |
Pos 3/7 (42.86%) |
Pos 3/7 (42.86%) |
| Neg 2/7 (28.57%) |
Neg 4/7 (57.14%) |
Neg 0/7 (0%) |
Neg 3/7 (42.86%) |
Neg 5/7 (71.43%) |
Neg 2/7 (28.57%) |
Neg 4/7 (57.14%) |
Neg 4/7 (57.14%) |
Neg 4/7 (57.14%) |
|
| 2 Segments 23/32 (71.88%) | Pos 14/23 (60.87%) |
Pos 9/23 (39.13%) |
Pos 5/23 (21.74%) |
Pos 11/23 (47.83%) |
Pos 6/23 (26.09%) |
Pos 6/23 (26.09%) |
Pos 13/23 (56.52%) |
Pos 12/23 (52.17%) | Pos 11/23 (47.83%) |
| Neg 9/23 (39.13%) |
Neg 14/23 (60.87%) |
Neg 5/23 (21.74%) |
Neg 12/23 (52.17%) | Neg 17/23 (73.91%) | Neg 12/23 (52.17%) |
Neg 10/23 (43.48%) | Neg 11/23 (47.83%) |
Neg 9/23 (39.13%) |
|
| 1 Segment 2/32 (6.25%) | Pos 2/2 (100%) |
Pos 2/2 (100%) |
Pos 2/2 (100%) |
Pos 1/2 (50%) |
Pos 1/2 (50%) |
Pos 1/2 (50%) |
Pos 1/2 (50%) |
Pos 1/2 (50%) |
Pos 1/2 (50%) |
| Neg 0/2 (0%) |
Neg 0/2 (0%) |
Neg 0/2 (0%) |
Neg 1/2 (50%) |
Neg 1/2 (50%) |
Neg 1/2 (50%) |
Neg 1/2 (50%) |
Neg 1/2 (50%) |
Neg 1/2 (50%) |
|
| Negative 4/36 (11.11%) | Pos 3/4 (75%) |
Pos 2/4 (50%) |
Pos 2/4 (50%) |
Pos 1/4 (25%) |
Pos 0/4 (0%) |
Pos 0/4 (0%) |
Pos 2/4 (50%) |
Pos 1/4 (25%) |
Pos 1/4 (25%) |
| Neg 1/4 (25%) |
Neg 2/4 (50%) |
Neg 1/4 (25%) |
Neg 3/4 (75%) |
Neg 4/4 (100%) |
Neg 3/4 (75%) |
Neg 2/4 (50%) |
Neg 3/4 (75%) |
Neg 2/4 (50%) |
|
Pos–positive; Neg—negative