To examine kidney function, albuminuria and creatinine levels were measured by ELISA using 24-hour urine collection. (a, b) Elevated levels of albuminuria and urine albumin/creatinine ratio (ACR) in HFD WT were significantly improved by CCR2 depletion. (c) Representative images of H&E-stained kidney sections. (d) Glomerular volume was measured by Image analysis software. Increased glomerular volume in HFD WT mice was significantly restored by CCR2 depletion in HFD KO. Original magnification is x400; bar = 50 μm. RD WT, Regular diet wild type; RD KO, Regular diet CCR2 knockout; HFD WT, High-fat diet wild type; HFD KO, High-fat diet CCR2 knockout. *P < 0.05 compared to that in RD WT; #P < 0.05 compared to that in HFD WT.