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. 2019 Sep 9;14(9):e0221048. doi: 10.1371/journal.pone.0221048

Fig 4. EV71 3Cpro inhibits binding of hnRNP A1 to the apaf-1 5’UTR.

Fig 4

(A) Binding of hnRNP A1 to the apaf-1 5’UTR is specific. Radiolabeled apaf-1 5’UTR was incubated at 37°C for 30 min either alone (lane 1) or with 1 μg of purified recombinant hnRNP A1 (lanes 2–6). Lanes 3–6: 1x, 2x, 4x, and 8x molar excess of unlabeled apaf-1 5’UTR RNA were added to the reactions as a specific competitor. (B) 1x, 2x, 4x, and 8x molar excess of Sindbis virus RNA, which does not interact with hnRNP A1, were added to the binding reactions as a non-specific competitor RNA (lanes 3–6). (C) Effect of 3Cpro on hnRNP A1–apaf-1 5’UTR binding. EMSA was performed as described above following the addition of wild-type or mutant recombinant 3Cpro to RNA-protein binding reactions, as indicated.