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. Author manuscript; available in PMC: 2019 Sep 9.
Published in final edited form as: Cell Rep. 2019 Aug 27;28(9):2256–2263.e3. doi: 10.1016/j.celrep.2019.07.074

Figure 1. D1+ MSNs Projecting to VP and VTA Are Distinct Cell Populations.

Figure 1.

(A) Left: schematic and representative images for injections of CTB-647 into the VP (green) and CTB-488 into the VTA (blue) of D1-tdTomato mice (red) (scale bar, 500 μm). Right: confocal images of retrogradely labeled neurons in the NAcMS (arrows indicate dual-labeled MSNs; scale bar, 100 μm).

(B) Left: quantification of CTB and tdTomato overlap in the NAcMS (total cell count of three slices per mouse, five mice). Right: summary of the number of D1+VTA, D1+VP and dual-projecting MSNs in the NAcMS (average number of labeled cells of three slices per mouse, five mice).

(C) Two-photon images of D1+VTA, D1+VP, and D1− MSNs (left) (scale bar, 20 μm) and quantification of total dendritic length for all reconstructed cells (right), showing no differences.

(D) Examples of physiological responses of D1+VTA, D1+VP, and D1− MSNs to current injections of 250 and −50 pA. Right: summary of current step-evoked firing, showing similar number of action potentials (APs) for D1+VTA and D1+VP MSNs.

Box-and-whisker plots represent median and minimum to maximum. F-I curve is presented as mean ± SEM. *p < 0.05.

See also Figure S1.