Figure 2.
(A) Cell-specific induction of PSAT1 mRNA by 3OH-pyruvate. REC or MIO-M1 cells were treated overnight with 1 mM 3OH-pyruvate or glyoxylate, total RNA was isolated and analyzed for PSAT1 mRNA normalized to HPRT1 mRNA by RT-qPCR and expressed as fold-change versus untreated controls (n = 5 for each condition). (B) Increased αKG/succinate ratio in 3OH-pyruvate treated REC cells measured by GC-MS (see Methods for the details). Ctr, control; 3OH-Pyr, 3OH-pyruvate; Pyr, pyruvate (n = 6 for each condition). *P < 1 × 10−5; **P < 5 × 10−6 versus control.