Figure 6.

MAP7 binds to the acetylated region of microtubules. A, Fluorescence and merged images of a region of a control COS cell transfected with EGFP and labeled for acetylated (acetyl; red) and tyrosinated (tyros; magenta) microtubules (arrows). B, Line scan along a single microtubule comparing acetylated and tyrosinated tubulin levels in (A). C, Fluorescence or merged images of a region of a COS cell expressing MAP7 (green) and labeled for α-tubulin (cyan). Arrows identify microtubule regions lacking MAP7-EGFP. D, Line scans of MAP7 and α-tubulin signals along a single microtubule from (C). E, Fluorescence (top rows) or merged (bottom rows) images of a region of a COS cell transfected with MAP7-EGFP (green) and labeled for acetylated (acetyl; red) and tyrosinated (tyros; magenta) tubulin. Arrows point to regions of microtubules positive for tyrosinated tubulin but lacking both MAP7-EGFP and acetylated-tubulin signals. F–H, Line scans along a single microtubule comparing the spatial relationship of fluorescent signals between MAP7-EGFP and acetylated (F) or tyrosinated tubulin (G) or between acetylated and tyrosinated tubulin (H). Scale bars: 5 μm.