Table 1.
Key resources, suppliers, and unique identifying information
| Reagent or Resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-DsRed | Clontech Laboratories | Catalog #632496, RRID:AB_10013483 |
| Goat anti-rabbit polyclonal AlexaFluor 594 | ThermoFisher Scientific | Catalog #A-11037, RRID:AB_2534095 |
| Chemicals, peptides, and recombinant proteins | ||
| Collagenase type P | Sigma-Aldrich | Catalog #11249002001 |
| OCT compound | Tissue-Tek | Catalog #4583 |
| Trypsin-EDTA 0.05% | Fisher Scientific | Catalog #25300054 |
| Fluoromount-G | ThermoFisher Scientific | Catalog #00-4958-02 |
| Laminin | Sigma-Aldrich | Catalog #L2020–1MG |
| MEM | Fisher Scientific | Catalog #11098050 |
| Penicillin-streptomycin | Fisher Scientific | Catalog #15-140-122 |
| MEM vitamin solution | ThermoFisher Scientific | Catalog #SH3059901 |
| B-27 supplement | Fisher Scientific | Catalog #17504044 |
| Horse serum, heat inactivated | ThermoFisher Scientific | Catalog #26050070 |
| Fura-2, AM | ThermoFisher Scientific | Catalog #F1221 |
| Pluronic F-127 | ThermoFisher Scientific | Catalog #P3000MP |
| Human NaV1.7 plasmid | Dr. Manu Ben-Johny | |
| DMEM | ThermoFisher Scientific | Catalog #11995073 |
| Fetal bovine serum | ThermoFisher Scientific | Catalog #A3840101 |
| TTX | Abcam | Catalog #ab120054 |
| AH-TTX | R&D Systems | Catalog #6159/100U |
| PF 05089771 | Tocris Bioscience | Catalog #5931 |
| ICA 121431 | Tocris Bioscience | Catalog #5066/10 |
| PN3a | Deuis et al. (2017) | |
| Critical commercial assays | ||
| Plasmid Maxi kit | Qiagen | Catalog #12162 |
| RNAscope Fluorescent Multiplex kit | Advanced Cell Diagnostics | Catalog #320850 |
| Deposited data | ||
| Source Data (freely available through Mendeley) | This study | http://dx.doi.org/10.17632/bxsvmy2zh3.1 |
| Movie of calcium imaging experiment | This study | http://dx.doi.org/10.17632/bxsvmy2zh3.1 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Slc17a8iCre: Tg(Slc17a8-icre)1Edw/SealJ | The Jackson Laboratory | RRID:IMSR_JAX:018147 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | RRID:IMSR_JAX:007914 |
| Homo sapiens: embryonic kidney cells stably transfected with NaV1.1, β1 and β2 subunits | Kahlig et al. (2010) | |
| Homo sapiens: embryonic kidney cells stably transfected with NaV1.6 | Dr. Lori Isom | |
| Oligonucleotide | ||
| Trpm8 probe channel 3 | Advanced Cell Diagnostics | Catalog #420451-C3 |
| Scn1a probe channel 2 | Advanced Cell Diagnostics | Catalog #434181-C2 |
| Scn8a probe channel 2 | Advanced Cell Diagnostics | Catalog #434191-C2 |
| Scn9a probe channel 2 | Advanced Cell Diagnostics | Catalog #313341-C2 |
| Scn10a probe channel 2 | Advanced Cell Diagnostics | Catalog #426011-C2 |
| Software and Algorithms | ||
| pClamp 10 Software Suite | Molecular Devices | https://www.moleculardevices.com |
| Image J | Schneider et al. (2012) | https://imagej.nih.gov/ij/index.html |
| MATLAB 2017b | MathWorks | https://www.mathworks.com |
| Software, algorithm, custom (MATLAB) | This study | https://github.com/thelumpkinlab/Calcium-Imaging-Analysis-Script |
| MetaMorph v7.50.6 | Molecular Devices | https://www.moleculardevices.com |
| MetaFluor v7.60.3 | Molecular Devices | https://www.moleculardevices.com |
| Prism v7.0b | GraphPad | https://www.graphpad.com |