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. 2019 Sep 5;8:e45590. doi: 10.7554/eLife.45590

Figure 3. TRPC3 contributes to the in vivo simple spike firing rate of Z–, but not Z+ PCs.

(A) Schematic illustration of extracellular recording configuration in vivo. PF, parallel fiber; CF, climbing fiber; MF, mossy fiber; GC, granule cell. (B) Representative sagittal cerebellar section with recording sites labeled by BDA injection, in lobule II (black arrow) and X (white arrow). (C) Representative example traces (left) and ISI distributions (right) of a Z– PC (top) and a Z+ PC (bottom) in gain-of-function TRPC3Mwk/- mice. (D) PC simple spike firing rate recorded in vivo in TRPC3Mwk/- mice compared to control littermates, for the Z–lobules I-III (light-red, n = 36/N = 7 mutants vs. n = 40/N = 6 controls, t60 = −4.58, p<0.001) and the Z+ lobule X (dark-red, n = 20/N = 6 mutants vs. n = 24/N = 5 controls, t42=-1.47, p=0.148). (E) Representative example traces (left) and ISI distributions (right) in a Z– PC (top) and a Z+ PC (bottom) of loss-of-function pcp2Cre;TRPC3fl/fl mice. (F) PC simple spike firing rate of pcp2Cre;TRPC3fl/fl mice compared to controls, for Z– lobules I-III (light-green, n = 30/N = 7 mutants vs. n = 26/N = 8 controls, t54=2.88, p=0.006) and in Z+ lobule X (dark-green, n = 32/N = 8 mutants vs. n = 24/N = 6 controls, t54 = −0.053, p=0.958). Data are represented as mean ± s.d., for values see Source data, ** means p<0.01 and ***p<0.001.

Figure 3—source data 1. Source data for Figure 3 and supplement.
Source data for the in vivo recordings in TRPC3Mwk/- and pcp2Cre;TRPC3fl/fl mice, simple spikes data.
elife-45590-fig3-data1.xlsx (34.4KB, xlsx)
DOI: 10.7554/eLife.45590.012

Figure 3.

Figure 3—figure supplement 1. In vivo extracellular recordings of PC simple spike activity in TRPC3Mwk/- and pcp2Cre;TRPC3fl/fl mice.

Figure 3—figure supplement 1.

(A–B) CV, a measure for regularity of the entire trace, for simple spikes (SS-CV) of PCs recorded in TRPC3Mwk/- mice, were significantly increased in both Z– and Z+ PCs. In Z– PCs this can exclusively be attributed to TRPC3 gain-of-function, while in Z+ PCs the loss of the regular input from UBCs (predominantly present in Z+ areas) potentially contributes to the phenotype. CV2, a measure for regularity on short time-scales, for simple spikes (SS-CV2), in both Z– and Z+ PCs of TRPC3Mwk/- mice were unaffected, compared with those of littermate controls. (C–D), In pcp2Cre;TRPC3fl/fl mice, SS-CV, SS-CV2 in both Z– and Z+ PCs do not differ from littermate controls. Error bars denote s.d.. See Source data for values and statistics, * equals p<0.05, **p<0.01 and ***p<0.001.