Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | ATPalpha1 | NA | FLYB:FBgn0027548 | |
| Strain, strain background (D. melanogaster) | ATPalpha1 △2-6b founder line, w[1118];;ATPa△2-6b attP/TM6B,Tb[1] | this paper | N/A | available on request |
| Strain, strain background (D. melanogaster) | 15 engineered variants of ATPalpha1 | this paper | N/A | See Supplementary file 2, available on request |
| Genetic reagent (D. melanogaster) | y[1], w[67c23], P{y[+mDint2]=Crey}1b;;D[*]/TM3, Sb[1] | Bloomington Drosophila Stock Center | BDSC:851 FLYB:FBst0000851; RRID:BDSC_851 | Source of Cre protein |
| Genetic reagent (D. melanogaster) | w[*];;ry[506] Dr[1]/TM6B, P{w[+mC]=Dfd-EYFP}3, Sb[1],Tb[1],ca[1] | Bloomington Drosophila Stock Center | BDSC:8704 FLYB:FBst0008704; RRID:BDSC_8704 | Balancer, chr 3 |
| Sequence-based reagent | PCR/sequencing primers, Sanger-based sequencing | this paper | N/A | ATPa1 H1-H2 Forward: AAAACTTGGAGCGCGATGGT; ATPa1 H1-H2 Reverse: ATACGGTCGCCGAACTTCAC |
| Sequence-based reagent | PCR/sequencing primers for Illumina-based sequencing | this paper | N/A | ATPa1 H1-H2 Forward 2834: GTCTCGTGGGCTCGGCGCTTTCAGACACATCCCGA; ATPa1 H1-H2 Forward 3185: TCGTCGGCAGCGTCTGAGAAAATGCCCGTCACGA |
| Sequence-based reagent | PCR-added linker primers, Illumina-based sequencing | this paper | N/A | ATPa1 H1-H2 i5: AATGATACGGCGACCACCGAGATCTACACnnnnnnnnTCGTCGGCAGCGTCA; ATPa1 H1-H2 i7: CAAGCAGAAGACGGCATACGAGATnnnnnnnnGTCTCGTGGGCTCGG; where ‘nnnnnnnn’=any sequence index |
| Chemical compound, drug | Ouabain | Sigma | Cat#: O3125 | |
| Chemical compound, drug | Porcine sodium potassium ATPase, cerebral cortex | Millipore-Sigma | Cat#: A7510 | |
| Recombinant DNA reagent | pGX-attB-ATPa2-6b | this paper | N/A | available on request |
| Software, algorithm | PAML v4.8 | PMID: 17483113 | RRID:SCR_014932 | http://abacus.gene.ucl.ac.uk/software/paml.html |
| Software, algorithm | BayesTraits v3.01 | PMID: 16685633 | RRID:SCR_014487 | http://www.evolution.rdg.ac.uk/BayesTraitsV3.0.1/BayesTraitsV3.0.1.html |
| Other | multiple sequence alignment (protein) | this paper | N/A | Supplementary file 3 |
| Other | multiple sequence alignment (nucleotide) | this paper | N/A | Supplementary file 4 |