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. 2019 Sep 9;8:e48417. doi: 10.7554/eLife.48417

Figure 2. Viral transduction in human brain slice cultures and 3D reconstruction of GFP-labeled pyramidal neurons.

Figure 2.

(A) Representative example of a human brain slice after viral transduction with AAVrg-hSyn-GFP at 9 DIV, scale bar 1000 µm. (A1) Enlarged confocal image from A: layers 2/3 pyramidal neurons with intact apical dendrites (red arrows) and axons (magenta arrows), scale bar 200 µm. (A2) The soma and axon initial segment (AIS) are clearly visible in the virally transduced neurons, scale bar 50 µm. (B) 3D reconstructions of four GFP transduced pyramidal neurons were performed from confocal z-stack tile scans, scale bar 200 µm. The cells were individually traced and pseudo colored (C). (B1) Example of two neurons within close proximity of each other, which could still be clearly separated for further analysis, scale bar 50 µm. (D) Separation of the four distinct GFP-labeled pyramidal cells for further analysis, scale bar 200 µm.