Skip to main content
. 2019 Sep 9;8:e48417. doi: 10.7554/eLife.48417

Figure 6. Presence and functionality of interneurons in human slice cultures.

Figure 6.

(A) Example of Layers 2/3 basket cell labled with biocytin and (B) after reconstruction, both scale bars 200 µm. (C) Staining of calretinin revealed presence of a subpopulation of inhibitory interneurons in acute (0 DIV) and late in culture (9 DIV), scale bar 20 µm. (D) Example of fast spiking interneuron firing in acute slices (orange) and late (blue) in culture (13 DIV). (E) Examples of interneuron APs in acute slice (orange) and late in culture (13 DIV, blue) reveal comparable AP half width. (F) Quantification and plotting of basic properties of IN in relation to the DIV. (G) Group comparison between the properties in acute slice and late in culture measured interneurons revealed no significant differences (Mann-Whitney test, p>0.05).