Table 3. Plasmids used in this work.
| Name | Description | Origin/ Marker |
Reference |
|---|---|---|---|
| pSP60 −2 | pSP60 carrying the counter selection cassette rpsL-erm | pSC101*/ AmpR |
(Dailidiene et al., 2006; Pernitzsch et al., 2014) |
| pA3-Up WT | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus amplified with the FA406/FA386 primer pair | ColE1/ AmpR |
This study |
| pA3-Down WT | pGEM-T carrying the downstream fragment of the aapA3/IsoA3 locus amplified with the FA409/FA387 primer pair | ColE1/ AmpR |
This study |
| pA3-Down pIsoA3* | pGEM-T carrying the downstream fragment of the aapA3/IsoA3 locus containing IsoA3 −10 box inactivated (A87C/A90G) | ColE1/ AmpR |
This study |
| pA3-Up start | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the AapA3 start codon mutation G54T | ColE1/ AmpR |
This study |
| pA3-Up A28C | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the suppressor A28C | ColE1/ AmpR |
This study |
| pA3-Up A33T | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the A33T mutation | ColE1/ AmpR |
This study |
| pA3-Up A40T | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the suppressor A40T | ColE1/ AmpR |
This study |
| pA3-Up A40T/A33T | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the A40T and the compensatory mutation A33T | ColE1/ AmpR |
This study |
| pA3-Up T78C | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the suppressor T78C | ColE1/ AmpR |
This study |
| pA3-Down T78C | pGEM-T carrying the downstream fragment of the aapA3/IsoA3 locus containing the suppressor T78C | ColE1/ AmpR |
This study |
| pA3-Down T78C/pIsoA3* | pGEM-T carrying the downstream fragment of the aapA3/IsoA3 locus containing the suppressor T78C and IsoA3 −10 box inactivated (A87C/A90G) | ColE1/ AmpR |
This study |
| pA3-Up G43A | pGEM-T carrying the upstream fragment of the aapA3/IsoA3 locus containing the SD suppressor G43A | ColE1/ AmpR |
This study |
*Nucleotide positions are indicated relative to the AapA3 transcriptional start site (TSS, +1).