Table 4. Escherichia coli strains used in this work.
| Name | Description/genotype | Plasmid | Resistance | Reference |
|---|---|---|---|---|
| TOP10 | mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG | none | none | Thermo Fisher Scientific, Invitrogen |
| A3-Up WT | TOP10 | pA3-Up WT | AmpR | This study |
| A3-Down WT | TOP10 | pA3-Do WT | AmpR | This study |
| A3-Down pIsoA3* | TOP10 | pA3-Do pIsoA3* | AmpR | This study |
| A3-Up start | TOP10 | pA3-Up start | AmpR | This study |
| A3-Up A28C | TOP10 | pA3-Up A28C | AmpR | This study |
| A3-Up A33T | TOP10 | pA3-Up A33T | AmpR | This study |
| A3-Up A40T | TOP10 | pA3-Up A40T | AmpR | This study |
| A3-Up A40T/A33T | TOP10 | pA3-Up A40T/A33T | AmpR | This study |
| A3-Up T78C | TOP10 | pA3-Up T78C | AmpR | This study |
| A3-Down T78C | TOP10 | pA3-Do T78C | AmpR | This study |
| A3-Down T78C/pIsoA3* | TOP10 | pA3-Do T78C/pIsoA3* | AmpR | This study |
| A3-Up G43A | TOP10 | pA3-Up G43A | AmpR | This study |
*Nucleotide positions are indicated relative to the AapA3 transcriptional start site (TSS, +1).