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. Author manuscript; available in PMC: 2019 Sep 9.
Published in final edited form as: Sci Transl Med. 2019 Feb 27;11(481):eaat2004. doi: 10.1126/scitranslmed.aat2004

Fig. 2. Children and adults differ in IgG and IgA subclass usage but show comparable IGHV gene selection correlated with isotype switching.

Fig. 2.

(A) The fraction of each IgG or IgA subclass used is shown. STORK specimen samples (n=89) are indicated as points in the age one to three year category, and the 114 healthy adults (age 17–87 years) are the same as those in Fig. 1. The sum of the subclass fractions is more than 1.0 because a clone can contain members expressing different subclasses. p values were determined by two-sided Wilcoxon-Mann-Whitney tests: *p < 0.05, **p < 0.01, and ***p < 0.001. IGHV gene usage is shown as the average for clones within each (B) child or (C) adult. Isotypes are plotted in their chromosomal ordering, from upstream isotypes IgM and IgD to the most downstream isotype, IgA2. IGHV ordering is based on the 20 most common IGHV genes in IgM in the STORK subjects. IgG4 and IgE are excluded due to low frequency. Dots represent outliers beyond 1.5-fold of the interquartile range. The plot axes were chosen to show the box-whiskers on a readable scale; rare outlier points with extreme values are not shown, but were included in all analyses.