Skip to main content
. Author manuscript; available in PMC: 2019 Sep 9.
Published in final edited form as: Pharmacol Res. 2019 Jul 21;147:104329. doi: 10.1016/j.phrs.2019.104329

Fig. 3. Endothelium-dependent and -independent relaxation induced by TRPM8 activation in IPA.

Fig. 3.

A. The concentration–response curves of icilin in the presence (●, intact endothelium, n = 6) or in the absence of functional endothelium (O, Endothelium denuded, n = 6). The response is expressed as a percentage of relaxation from Phenylephrine-induced contraction. B. Logarithmic concentration response curves to icilin obtained from studies with IPA pre-contracted by Phe (10−6 M), with functional endothelium, in the absence (●, control, n = 6) or in the presence of indomethacin (▼, 10−5 M), n = 6), L-NAME (■, 10−4 M, n = 6) or a combination of charybdotoxin (10−7 M) and apamin (5×10−6 M) (▲; n = 5). C. Logarithmic concentration-response curves showing the relaxant effect of icilin, in the IPA pre-contracted by Phe (10−6 M) without functional endothelium, in the absence (●, endothelium denuded, n = 6) or in the presence of iberiotoxin (▼, 10−7 M, n = 4) or TEA (■, 3 × 10−3 M, n = 6). Values are expressed as means ± S.E.M. The data were examined using unpaired Student’s t tests (Fig. 3A) and one-way ANOVA followed by the Bonferroni post-test (Fig. 3B and 3C). *p < 0.05 and ***p < 0.001 vs control.