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. Author manuscript; available in PMC: 2019 Sep 9.
Published in final edited form as: Pharmacol Res. 2019 Jul 21;147:104329. doi: 10.1016/j.phrs.2019.104329

Fig. 4. TRPM8 channels activation seems inhibit RhoA/ROCK kinase pathway.

Fig. 4.

A. Logarithmic concentration response curves to icilin obtained from studies with IPA pre-contracted by Phe (10−6 M), without functional endothelium, in the absence (▲, control, n = 6) or in the presence of Y27632 (●, 10−6 M, n = 6). B and C. Protein expression analysis for RhoA and ROCKII normalized by GAPDH, in VSMCs from IPA, incubated in either vehicle (V, n = 5), Phenylephrine (P, 10−6 M, n = 5), Phenylephrine + icilin (10−5 M, P + I, n = 5) or Phenylephrine + icilin + BCTC (2 × 10−6 M, P + I + B, n = 5). Above, densitometric analysis; below, representative images of immunoblots. The data were examined using unpaired Student’s t tests (Fig. 4A) and one-way ANOVA followed by the Bonferroni post-test (Fig. 4B and 4C).