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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Biochem Pharmacol. 2019 Jun 14;168:1–13. doi: 10.1016/j.bcp.2019.06.010

Figure 3. Increased Nodal expression in Akt1-deficient PCa cells is attenuated by treatment with FoxO inhibitor AS1842765.

Figure 3

(A-B) Representative Western blot images and bar graph of the band densitometry analysis of ShControl and ShAkt1 DU145 and PC3 cells probed for phosphorylated and total expression of FoxO1 and FoxO3a showing a significant decrease in FoxO phosphorylation in ShAkt1 PCa cells compared to ShControl, respectively (n=6). (C-D) Representative Western blot images and bar graph of the band densitometry analysis of ShControl and ShAkt1 DU145 and PC3 cells probed for Nodal expression showing significant increase in Nodal expression in ShAkt1 PCa cells and a significant decrease in Nodal expression in ShAkt1 PCa cells by treatment with FoxO1/3a inhibitor AS1842765 (10 µM; 72 hours; n=3). Data are presented as mean ± SEM. *p<0.05; **p<0.01; #p<0.001.