Table 1.
Cross-linking propensity of PDE6 intra-subunit cross-links.
| Lys 1 | Lys 2 | Holoenzyme (%XL) | Pαβγ2-cGMP (%XL) | Pαβ (%XL) | Pαβγ2 (%XL) | Pαβ-cGMP (%XL) |
|---|---|---|---|---|---|---|
| Pα | ||||||
| 76 | 84 | 27.7±4.3 | 19.1±2.3 | 8.2±2.0* | 23.2±1.4 | 21.6±9.9 |
| 534 | 581 | 16.7±1.1 | 17.7±0.6 | 23.6±0.9** | 21.6±2.8 | 17.1±2.5 |
| 677 | 683 | 40.4±3.1 | 44.7±0.7 | 51.9±4.1* | 36.4±0.8** | 52.8±5.4** |
| 765 | 819 | 1.3±0.3 | 3.7±0.6 | 3.5±0.2 | 1.5±0.2** | 5.1±0.8** |
| 765 | 827 | 7.7±0.1 | 15.7±1.1 | 15.0±0.1 | 6.5±0.5** | 24.6±1.8** |
| 819 | 827 | 62.6±1.2 | 60.3±2.6 | 59.8±0.5 | 66.7±5.7 | 53.6±0.7 |
| Pβ | ||||||
| 490 | 532 | 7.1±2.4 | 8.0±0.9 | 4.8±1.2 | 6.2±2.6 | 8.1±1.9 |
| 675 | 681 | 30.3±4.1 | 30.0±2.1 | 46.4±0.7** | 21.8±1.9* | 50.2±8.0** |
| 763 | 826 | 2.6±0.1 | 2.6±0.4 | 2.4±0.6 | 1.8±0.2 | 2.9±0.1 |
| 763 | 827 | 1.2±0.4 | 0.7±0.1 | 0.8±0.1 | 0.5±0.1 | 0.9±0.1 |
| 763 | 817 | 14.0±1.5 | 36.6±1.0 | 32.9±3.1 | 19.9±0.4** | 47.9±1.0** |
| 763 | 832 | 1.9±0.3 | 0.2±0.0 | 0.3±0.0 | 0.1±0.1 | 0.3±0.0 |
| 817 | 827 | 10.5±0.3 | 6.5±1.3 | 6.9±2.0 | 7.4±1.0 | 4.9±0.8 |
| 817 | 826 | 28.8±3.6 | 29.3±1.1 | 31.3±1.1* | 37.2±0.1** | 23.2±0.4** |
| 817 | 832 | 9.6±0.4 | 3.2±0.8 | 2.3±0.0 | 4.1±0.6 | 4.8±0.8* |
| 826 | 832 | 0.6±0.1 | 3.2±0.3 | 3.3±0.1 | 3.1±0.2 | 2.9±0.3 |
Statistical significance was evaluated by ANOVA analysis followed by Tukey’s test. Conformational states that significantly deviated from the reconstituted PDE6 holoenzyme (Pαβγ2-cGMP) are annotated by asterisks (** for 0.05 α value; * for 0.1 α value).