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. Author manuscript; available in PMC: 2020 Sep 6.
Published in final edited form as: J Mol Biol. 2019 Jul 19;431(19):3706–3717. doi: 10.1016/j.jmb.2019.07.025

Figure 3. 2D reconstruction of KRAS-GppNHp proteins assembled on membranes.

Figure 3.

2D crystals of KRAS-GppNHp proteins assembled on PS-containing membranes were prepared, processed and imaged described in the Methods section. From a set of seven crystals, Fourier transform amplitude and phase data were merged assuming p3 symmetry. The averaged unit cell was a = b = 97.9 ± 5.0 Å, γ = 120.0 + 4.8°. The merge was performed to a resolution of 20Å using reflections of IQ≤6, and the phase residual for the merge was 8.1°, where 0° indicates perfect matching, and 90° indicates random matching. Protein areas appear white, and are viewed perpendicular to the membrane. Bright and faint trimer units respectively are outlined with thick- and thin-lined triangles.