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. 2019 May 13;27(6):803–816. doi: 10.1016/j.jsps.2019.05.002

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Representive anti-glucagon immunohistochemistry in the islets of langerhans in the different experimental groups (X400), (A) normal control showing normal distribution of gulcagon in peripherally located α-cells of islets (arrow); (B) diabetic control showing marked increase of the glucagon content of the α-cells in both central (arrow) and peripheral areas (C) GLB and (D) MCFE-250 showing mild elevation in glucagon content of the α-cells (arrow); (E) MCFE-500; (F) GLB plus MCFE-250 and (G) GLB plus MCFE-500 showing marked reduction in glucagon content of the α-cells in central area (arrow); (H) The bar chart represents anti-glucagon immunopositive staining expressed as area %. Values with different superscripts are significantly different (p ≤ 0.05).