Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 9;10:4093. doi: 10.1038/s41467-019-12104-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — OLPs receive presynaptic inputs predominantly from Rh6-PRs. a, b The contribution of Rh5- and Rh6-PRs to light-evoked calcium responses in OLPs as revealed by stimulation at different wavelengths in wild-type and Rh6 mutants. Left: schematic diagram illustrating the stimulation scheme used in calcium imaging experiments. Green or blue light pulses (dashed lines, green: 561 nm, blue: 488 nm) activate Rh5- or Rh6-PRs and elicit OLP-LexA-driven GCaMP6f signals in the somas of OLPs. Right: Representative raw traces of OLP > GCaMP6f collected from wild-type and Rh6 mutants (rh6^−/−). Magenta: cha-lOLP; blue: glu-lOLP; gray: pOLP. c, d OLPs are functionally connected to Rh6-PRs in the third instar larval brain. Light pulses (dashed lines, green: 561 nm, blue: 488 nm) induced fast calcium transients in cha-lOLP (magenta) and slow transients in glu-lOLP (blue) and pOLP (gray). Compared to wild-type controls, OLPs in Rh6 mutants showed no response towards green light (561 nm) stimulation and dampened responses toward blue light (488 nm) stimulation except for glu-lOLP, which remained equally responsive. The c average traces and d quantification of peak value of changed intensity (ΔF/F) are shown. Shaded areas on traces and error bars on quantifications represent SEM. Wild-type control: cha-lOLP, n = 15; glu-lOLP, n = 13; pOLP, n = 15. Rh6 mutant (rh6^−/−): cha-lOLP, n = 9; glu-lOLP, n = 7; pOLP, n = 7. cha-OLP, 561 nm: p < 0.0001, t = 5.102, df = 22; cha-OLP, 488 nm: p = 0.0007, t = 3.929, df = 22; glu-OLP, 561 nm: p = 0.0009, t = 3.977, df = 18; glu-OLP, 488 nm: p = 0.2362, t = 1.225, df = 18; pOLP, 561 nm: p = 0.0044, t = 3.207, df = 20; pOLP, 488 nm: p = 0.0261, t = 2.402, df = 20. Statistical significance was determined by Student’s t test. p ≥ 0.05 was considered not significant (n.s.), ***p < 0.001, **p < 0.01, and *p < 0.05