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. 2019 Sep 9;10(9):646. doi: 10.1038/s41419-019-1887-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Lack of marker expression for neural progenitors during the reprogramming process. The expression in neural stem cells (NSCs) served as a positive control. b Time-course analysis of proliferating cells by staining with PCNA (n = 20 randomly selected fields from triplicate samples). c, d Incorporation of BrdU in NGN2-induced neuronal cells. Cells were treated with BrdU for the indicated duration after viral infection (b). OM, OEC culture medium; NM, neuronal induction medium. *P < 0.05, ***P < 0.001 by Student’s t-tests. Scale bar = 50 μm