Fig. 8. Subtype analysis of NGN2-induced neurons from OECs.

a No expression of ChAT was detected in the induced neurons at 21 dpi. b, c Ectopic NGN2-induced OECs reprogramming into GABA⁺ (b) or vGlut1⁺ (c) neurons at 21 dpi. d Representative images of NGN2-induced GABA⁺ or vGlut1⁺ neurons at 40 dpi. e Quantification of the percentage of GFP⁺ cells expressing GABA or vGlut1 over the total of GFP⁺ cells (n = 20 randomly selected fields from triplicate samples). f Induction of GABAergic neurons from OECs of GAD67-GFP transgenic mice. OECs were cultured from olfactory bulb of adult GAD67-GFP transgenic mice, and then infected with NGN2-expressing lentivirus (without co-expressing GFP) to induce neuronal reprogramming. Immunocytochemical analysis was performed at 21 dpi. Scale bar = 50 μm