Table 1.

Oligonucleotides used to determine relative TLR7 and TLR8 copy numbers by real-time quantitative PCR assays.

Oligonucleotide	Sequence
TLR7 Forward	5′-CAGTATTGTGCTGTCTTTGAAATGTA-3′
TLR7 Reverse	5′-TGGTTGAAGAGAGCAGAGCA-3′
TLR7 Probe	5′-(FAM)TTGGGCCCATCTCAAGCTGATCTTG(TAMRA)-3′
TLR8 Forward	5′-GTGAGGCCACACAAGATGGA-3′
TLR8 Reverse	5′-TTCCAGACCACTCCCTTTGC-3′
TLR8 Probe	5′-(FAM)CGCCCAAGTGTCCACCTAAACATGAGT(TAMRA)-3′
HBD Forward	5′-AGATTCCTACTTTCAGCGTTGG-3′
HBD Reverse	5′-CAGCAGGGTTCAGGAAGATAAA-3′
HBD Probe	5′-(FAM)CAACCTGGATCCACTTGCCCAGTG(TAMRA)-3′
RPP30 Forward	5′-TTGTCGTTCAGAAGAAGACAAAGA-3’
RPP30 Reverse	5′-AGTTGACTAGGGATTCGGAGAAA-3′
RPP30 Probe	5′-(FAM)TGTTGATTTCAACACACAAATTCTGGTGG(TAMRA)-3′

Thermal cycling conditions were 95 °C for 10 minutes followed by 40 cycles at 95 °C for 15 seconds and 60 °C for 60 seconds.