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. 2019 Sep 9;9:12901. doi: 10.1038/s41598-019-49579-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Platform technology for ectopic antigen expression using BC-PIV. (a) Graphic depiction of the production of recombinant vaccines. Antigen genes are inserted into the cloning site (CS) 1 and/or CS2 of BC-PIV, and the resultant vectors are produced by the reverse genetics method^1–3,5. (b) Another method for antigen expression as a fusion protein with the C-terminus of HN of BC-PIV, and/or that of F from packaging cells. (c) A Western blot analysis of EBOV-GP on BC-PIV using 1 × 10⁶ particles/lane. The authentic or hybrid EBOV GP gene was inserted into CS2 of BC-PIV. F TM&CT, transmembrane and cytoplasmic tail regions of hPIV2 F. (d) A Western blot analysis of M2e peptide fused with hPIV2 HN and/or F on BC-PIV using 1 × 10⁶ particles/lane.