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. 2019 Sep 9;9:12901. doi: 10.1038/s41598-019-49579-y

Figure 2.

Figure 2

BC-PIV/EGFP-EBOV-GP proliferates only in the presence of hPIV2 F protein. The EGFP gene was inserted into the CS1 of BC-PIV/EBOV-GP and designated BC-PIV/EGFP-EBOV-GP. Vero/BC-F cells5, a packaging cell line stably expressing hPIV2 F protein, were infected with BC-PIV/EGFP-EBOV-GP at an MOI of 0.3. Vero cells were also infected with the same virus at an MOI of 0.3. BC-PIV/EGFP-EBOV-GP does not proliferate in Vero cells; however, it does proliferate in Vero/BC-F cells, resulting in a titer of 1 × 108 TCID50/mL. The EGFP expression was visualized by fluorescence microscopy (CKX41; OLYMPUS, Tokyo, Japan).