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. 2019 Sep 3;7:212. doi: 10.3389/fbioe.2019.00212

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Copyright © 2019 Zhang, Mohsin, Dai, Chen, Zhuang, Chu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The results of the developed breeding process. (A) Number of mutants selected in resistance screening. (B) Titer of salinomycin production by mutants grown in shaking flask culture. (C) Genetic stability of selected mutants after 3 generations. (D) Titer of salinomycin production by mutants TK, Tet³⁰Chl²⁵, and the initial strain S12 grown in shaking flask culture. Error bars indicate standard error of the mean.