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. 2019 Sep 3;7:208. doi: 10.3389/fbioe.2019.00208

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Copyright © 2019 Wanjare, Kawamura, Hu, Alcazar, Wang, Woo and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro characterization of engineered myocardial tissue composed of iCMs and iECs cultured in aligned or randomly oriented microfibrous scaffolds. Representative confocal images depict the attachment and cellular organization of iECs after 3 days of seeding, followed by the co-culture with iCMs for 2 more days. The iCMs were visualized by troponin-T (TNNT) expression (red), and iECs were visualized by CD31expression (green). Total nuclei were depicted by Hoechst 33342 dye (blue). Arrow denotes the orientation of the microfibers on aligned scaffolds. Scale bar: 100 mm.