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Berberine promoted the expression of pro-neural markers during the recovery period. (A) mRNA levels of pro-neural markers (ASCL1, NeuroG1, NeuroD2, and DCX) were detected in Step Two via real-time PCR analysis. (B) Western blot analysis of ASCL1, NeuroG1, NeuroD2, and DCX. (C–F) Quantification of protein blots. The data represent the mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
