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. 2019 Sep 3;13:395. doi: 10.3389/fncel.2019.00395

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Copyright © 2019 Shou, Cheung, Gao, Shi and Shaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Berberine induced neuronal differentiation of AAPH-damaged C17.2 cells. (A) Cell differentiation in group 1, group 2, group 3, and group 4. (B) Differentiation rate in group 1, group 2, group 3, and group 4. (C–G) mRNA levels of Nestin (C), MAP2 (D), TUBB3 (E), Olig2 (F), and GFAP (G) in Group 1, 2, 3, and 4. (H) Western blot analysis of Nestin, MAP2, TUBB3, Olig2, and GFAP in Group 1, 2, 3, and 4. (I–M) Quantification of protein blots. Group 1 was set as the control. The data represent the mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.