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. 2019 Sep 9;9:12908. doi: 10.1038/s41598-019-49145-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoprecipitation of FAK. Proteins immunoprecipitated from total cell lysate from the HCT-116 cell line using a polyclonal anti-FAK antibody or rabbit IgG as a negative control. Eluents were electrophoresed on 10% SDS-PAGE. (a) Silver staining of one gel demonstrated a 125 kDa band corresponding to FAK that was present in the anti-FAK immunoprecipitate HCT-116 lysates but not in the IgG control. (b) After SDS-PAGE, the gel was transferred to a nitrocellulose membrane and immunoblotted using FAK antibody. A band of 125 kDa corresponding to FAK was identified in the total cell lysate or anti-FAK IP but not in the IgG control.