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. 2019 Sep 9;10(9):647. doi: 10.1038/s41419-019-1876-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b The efficacy of LMCD1 specific shRNA was measured by qRT-PCR and western blotting. c ALP staining and Alizarin red staining were performed after treating BMSCs with osteogenic induction media for 7 days or for 14 days. d, e The expression of osteogenic markers was assessed by qRT-PCR and western blotting. f, g The efficacy of LMCD1 specific lentivirus was measured by qRT-PCR and western blotting. h ALP staining and Alizarin red staining were performed after treating BMSCs with osteogenic induction media for 7 days or for 14 days. i, j The expression of osteogenic markers was assessed by qRT-PCR and western blotting