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. 2019 Sep 9;9:12897. doi: 10.1038/s41598-019-49210-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TX-100 vesicle interactions reported using ensemble FRET and FCS. (a) FRET efficiency of Dil/DiD labeled vesicles versus TX-100. The solid line represents a Hill model fit. Inset: corresponding variation in fluorescence spectra. (b) Average lifetime of Dil versus TX-100. Inset: corresponding time-resolved fluorescence decays. Solid lines represent bi-exponential fits. (c) Top: FCS cross-correlation curves (solid lines), fits (dashed lines) (inset: zoomed in) associated with 100 nm-(black) and 200 nm-sized (red) vesicles. Also shown are FCS curves for 200 nm-sized vesicles in the presence of 0.1 mM (green) and 0.2 mM (blue) TX-100. Bottom: residuals of the fits. (d) Top: Diffusion times of NBD-PC labeled vesicles as a function of TX-100. A PC: PS lipid ratio of 65: 35 was used under all conditions. Solid lines represent linear fits. Bottom: corresponding residuals.