Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 9;9:12897. doi: 10.1038/s41598-019-49210-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Real-time visualization of solubilization kinetics by svFRET. (a) Schematic of the immobilization scheme. The schematic is for illustration purposes only and is not to scale. (b) Representative variation in the fluorescence emission of Dil and DiD (top panel), the sum of their intensities (middle panel) and the corresponding variation in FRET efficiency obtained before (<90 s) and after (>90 s) injection of 0.16 mM TX-100. (c) Relative FRET state occupancies observed over 1000 s. (d) FRET contour plot showing the variation in E_FRET before and after TX-100 injection (dashed white line) (N = 105). (e) Corresponding scatter plot of expansion half-live, t_E versus that of lysis, t_L obtained after injection of 0.08 mM (blue) and 0.16 mM (red) TX-100. Dashed lines represent the center of each distribution. (f) Comparative bar plots summarizing the variation in t_E and t_L as a function of TX-100 and percentage of cholesterol incorporated within the vesicle bilayer. Error bars indicate the standard error of the mean.