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. 2019 Sep 3;10:946. doi: 10.3389/fphar.2019.00946

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Copyright © 2019 Zhu, Dai, He, Xu and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell apoptotic analysis induced by Fe(PIP)₃SO₄. (A) Flow cytometric analysis for Annexin V–FITC/PI in U87 cells treated with Fe(PIP)₃SO₄ (5, 10, 20, and 40 μM, respectively) for 36 h. The percentage of Annexin V–positive and PI-negative cells (bottom right) represents the proportion of early apoptotic cells; the percentage of Annexin V–positive and PI-positive cells (top right) represents the proportion of late apoptotic cells. (B) Quantitative analysis of early apoptotic U87 cells induced by Fe(PIP)₃SO₄ (5, 10, 20, and 40 μM, respectively) after 36 h of incubation. Values are presented as means ± SD in triplicate. Significant difference between the treatment and control groups was indicated at the P<0.05 (*) or P<0.01 (**) level. (C) Quantitative analysis of late apoptotic U87 cells induced by Fe(PIP)₃SO₄ (5, 10, 20, and 40 μM, respectively) after 36 h of incubation. Values are presented as means ± SD in triplicate, and significant difference between the treatment and control groups is indicated at the P<0.05 (*) level.