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. 2019 Sep 3;10:775. doi: 10.3389/fgene.2019.00775

Figure 2.

Figure 2

Evaluation of predicted keratinocyte regulators via siRNA knockdown. (A) RNA was harvested 4 days after transfection from primary human keratinocyte culture treated with ETV4, ZBED2, or negative control siRNA. Quantitative polymerase chain reaction analysis showed significant (p < 0.05, Student t test) knockdown of ETV4 and ZBED2 mRNA relative to nontargeting siRNA transfected cells. (B) Expression of KRT10 and FLG transcript following siRNA knockdown of ETV4 or ZBED2, relative to control. Asterisks indicate p < 0.05 (Student t test). (C) same as (A) but for BNC1 and HOXC11. (D) Knockdown of BNC1 and HOXC11 did not significantly change expression of differentiation marker genes KRT10 and FLG. Error bars indicate 1 standard deviation calculated over four replicates.