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. 2019 Aug 15;7(4):223–231.

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Effect of Birc3 and ELL2 knockdown on LNCaP proliferation. (A) BrdU applied in LNCaP cells transfected with different nontarget siRNA (siCon), siELL2, siBirc3, or concurrent ELL2 and Birc3 knockdown (siDouble). Upper panel shows nuclear staining with Sytox Green (green), and lower panel shows BrdU-positive nuclei stained with CY3 (red). (B) Upper panel shows nuclear staining with DAPI (blue), and lower panel shows BrdU-positive nuclei stained with CY3 (red). (C) Quantification column graphs of BrdU experiment shown in (A) as mean precentage of BrdU-positive cells relative to the total number of cells. (D) Quantification of BrdU experiment shown in (B). (E, F) Knockdown efficiency confirmed by Western-blotting. The expression of Birc3 protein in LNCaP cell was suppressed by siRNA targeted to Birc3. GAPDH served as a loading control.