Figure 3.

Inhibition of WLS decreases Wnt signaling in MDVR cells. A. Western blots were used to assay for protein levels of WLS, LRP6, and phospho-LRP6 in MDVR cells transfected with WLS targeting siRNA (siWLS) or a non-targeting control siRNA (siC) for 72 hours. GAPDH served as a loading control. B. MDVR cells were transfected with siC or siWLS and harvested after 48 hours. qPCR demonstrates that siRNA mediated inhibition of WLS results in decreased expression of WLS, LEF1, and BAMBI in MDVR cells. * = p-value ≤ 0.05.