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. 2019 Aug 15;7(4):203–214.

Figure 4.

Figure 4

Inhibition of WLS attenuates cellular viability and induces apoptosis in enzalutamide-resistant prostate cancer cells. A. Cell viability assay demonstrates that siRNA-mediated inhibition of WLS (siWLS) versus non-targeting siRNA (siC) decreases MDVR cellular viability. B. PSA ELISA shows that inhibition of WLS reduces PSA secretion in MDVR cells. C. Colony formation assays demonstrate that inhibition of WLS reduces clonogenic potential of MDVR cells. D. MDVR cells were treated with siC or siWLS and harvested after 72 hours. Western blot for cleaved-PARP suggests induction of apoptosis in response to decreased WLS expression in MDVR cells. GAPDH served as a loading control. E. Western blot shows that Rv1 cells have increased WLS expression versus C4-2B cells. GAPDH served as a loading control. F. Cell viability assay demonstrates that siRNA-mediated inhibition of WLS decreases Rv1 cellular viability. G. Rv1 cells were treated with siC or siWLS and harvested after 72 hours. Western blot for cleaved-PARP suggests induction of apoptosis in response to decreased WLS expression in Rv1 cells. GAPDH served as a loading control. * = p-value ≤ 0.05.