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. 2019 Aug 15;7(4):203–214.

Figure 6.

Figure 6

WLS regulates AR expression and AR signaling. (A) MDVR cells were treated with non-targeting siRNA (siC) or WLS-targeting siRNA (siWLS) and harvested after 72 hours. Western blot shows decreased AR (top panel, light exposure) and AR-variant (AR-V) expression (lower panel, darker exposure) in response to siWLS in MDVR cells. Tubulin served a loading control. qPCR shows decreased (B). AR and AR-v7 expression and (C). PSA, NKX3.1, and UBE2C expression in response to siWLS in MDVR cells harvested 48 hours after treatment. (D) PSA ELISA with normalizing PSA by cell number demonstrates decreased PSA secretion in MDVR cells transfected with siWLS. (E) Western blot shows decreased AR and AR-variant expression in response to siWLS treatment in Rv1 cells. GAPDH served as a loading control. (F) qPCR shows decreased AR and AR-v7 expression in response to siWLS treatment in Rv1 cells. * = p-value ≤ 0.05.