Fig. 2.

Stromal prorenin receptor (PRR) is dispensable for the normal ureteric bud (UB) branching but important for normal lung development. A–C: Foxd1⁺ cells are observed in embryonic day (E)15.5 conditional deletion (cKO) and heterozygous (Het) kidneys as a honeycomb pattern that interdigitates between the forming nephron progenitor units from Cre-enhanced green fluorescent protein (eGFP) expression (green staining). Control (Con) kidney (Foxd1^Cre−/PRR^flox/flox) is eGFP negative. D and E: images of whole E15.5 kidneys show the number of UB tips (19 vs. 22, blue staining, anti-pancytokeratin) in E15.5 cKO and Con kidneys. F–M: on E18.5, Con lung has a well-developed aiway tree with widely opened lumens (arrows in F and J). cKO lung has poorly developed bronchial tree with narrow airway lumens; hematoxylin and eosin stain (F–I) and anti-pancytokeratin stain, red (J–M).