Fig. 2.

Elevated arterial shear rate increases endothelial nitric oxide (NO) synthase phosphorylation at serine 1177 (p-eNOS^S1177), NO generation, and O₂^·− production. Relative to values obtained before rhythmic handgrip exercise (Pre-HG), Post-HG endothelial cells (ECs) exhibited elevated p-eNOS^S1177 (A and C), NO generation (E), and O₂^·− production (F), whereas eNOS (B and D) protein expression was not altered by HG. Each panel presents a representative EC from each staining protocol: bottom left, vascular endothelial cadherin (VE-cadherin; green); bottom right, 4′,6′-diamidino-2-phenylindole (DAPI; blue); top left, protein of interest; and top right, merge. Mean intensity of the protein of interest (top left) is documented below each representative image. For A–D, n = 7 subjects. Note: two representative images are shown for p-eNOS^S1177 and eNOS. For E and F, n = 3 subjects. *P < 0.05 vs. Pre-HG. Scale bar represents 10 µm. Magnification = ×60. Symbols aligned with the histogram indicate individual subject data points. AU, arbitrary units; DAF, diaminofluorescein diacetate; DHE, dihydroethidium; HAEC, human arterial EC.