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(A) Mouse embryonic fibroblasts (MEFs) from C57BL/6, S100A16Tg/Tg and S100A16KO/+ mice were treated with cycloheximide (CHX) at 20 μM for different times (0, 12, or 24 h) and cell lysates were subjected to immunoblotting using anti-11β-HSD1 antibody. (B) Quantification of 11β-HSD1 expression described in (A) based on grayscale analysis. *P≤0.05 compared to controls.
