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. 2019 Aug 27;29:86–98. doi: 10.1016/j.molmet.2019.08.015

Figure 1.

Figure 1

CL treatment recruited macrophages that were closely associated with PDGFRα+ ASCs in gWAT. A. Immunostaining for F4/80 and PDGFRα in paraffin sections obtained from mice treated with CL for 3 days and vehicle control mice. B–C. Immunostaining of F4/80, neutral lipid staining, and Pdgfra-tdTomato fluorescence in cryosections obtained from tamoxifen induced PdgfraCreER/Rosa-LSL-tdTomato mice treated with CL for 3 days arrows indicated lipid+ adipocytes that were surrounded by F4/80+ macrophages. Arrow heads indicate newly generated multilocular adipocytes that were positive for PDGFRα+ reporter (tdTomato+). Nuclei were counterstained with DAPI. (size of bar = 20 μm) D–E. qPCR analysis of gWAT, iWAT, and BAT of mice treated with CL for 3 days and vehicle control mice (n = 6 per condition, mean ± S.E.M, **p < 0.01, ***p < 0.001).