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. 2019 Aug 27;29:86–98. doi: 10.1016/j.molmet.2019.08.015

Figure 6.

Figure 6

Overexpression of Rora in C3H10T1/2 cells inhibits adipogenesis. A. Putative miR-10a-5p binding site in Rora 3′UTR. B. Luciferase reporter assay of Rora mRNA 3′UTR. (mean ± S.E.M, Bonferroni posttests, n = 3 per group, **p < 0.01, ***p < 0.001) C. Lipid staining (BODIPY). C3H10T1/2 cells were infected and cultured with or without doxycycline (Dox, 2 μg/ml) during the course of differentiation. D. Western blot analysis. C3H10T1/2 were infected with doxycycline (dox) inducible pINDUCER21-RORA using lentivirus infection, and then cells were cultured with/without dox. miR-10a-5p mimics were treated before adipogenic differentiation. E. Anti-adipogenic role of Rora.