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. 2019 Sep 3;10:605. doi: 10.3389/fendo.2019.00605

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Copyright © 2019 Bai, Jiang, He, Chan and Wong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glucagon-induced IGF-I promoter activity expressed in αT3 cells. (A) Time course and (B) Dose dependence for glucagon induction of IGF-I promoter activity of carp origin. Transfection studies was performed in αT3 cells with pIGF1(-1077).Luc carrying a 1,077 bp 5′ promoter of the carp IGF-I gene. Glucagon treatment was initiated after 15 h incubation to allow for recovery after transfection. For time course experiment, the dose of glucagon used was fixed at 100 nM for the duration as indicated, while the duration of treatment was fixed at 24 h for the dose-response study. After glucagon treatment, cell lysate was prepared from αT3 cells and used for luciferase activity measurement.