Figure 2.

Glucagon-induced IGF-I, HNF1α, and CREB gene expression in primary culture of grass carp hepatocytes. (A) Time course and (B) Dose dependence of glucagon stimulation on IGF-I, HNF1α, and CREB mRNA expression in carp hepatocytes. For the time course experiment, the dose of glucagon used was fixed at 10 nM for the duration as indicated, while the duration of drug treatment was fixed at 12 h for the dose-response study. After glucagon induction, total RNA was isolated from individual wells and used for real-time PCR using primers for carp IGF-I, HNF1α, and CREB, respectively. Data presented are expressed as mean ± SEM and groups denoted by different letters represent a significant difference at p < 0.05 (ANOVA followed by Newman-Keuls test).