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Functional role of PKA and Epac activation on IGF-I, HNF1α, and CREB transcript expression at the hepatic level. Carp hepatocytes were treated for 12 h with increasing concentrations (0.1–100 nM) of the PKA-specific cAMP analog 6Bnz-cAMP or Epac-specific cAMP analog 8cpt-2Me-cAMP. After drug treatment, total RNA was isolated from individual wells and subjected to real-time PCR with primers for IGF-I, HNF1α, and CREB, respectively.
