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. 2019 Sep 3;10:605. doi: 10.3389/fendo.2019.00605

Figure 8.

Figure 8

Functional coupling of MAPK and PI3K/Akt cascades with PKA activation in IGF-I, HNF1α, and CREB mRNA expression at the hepatic level. Carp hepatocytes were exposed to the PKA-specific cAMP analog 6Bnz-cAMP (100 nM) for 12 h with co-treatment of (A) the MEK1/2 inhibitor PD98059 (10 μM) or ERK1/2 inhibitor SCH772984 (10 nM), or (B) the PI3K inhibitor Ly294002 (10 μM), Akt blocker API-2 (2 μM), or mTOR inactivator Rapamycin (20 nM). After drug treatment, total RNA was isolated from individual wells and used for real-time PCR with primers for IGF-I, HNF1α, and CREB, respectively.