Figure 9.
Functional coupling of MAPK and PI3K/Akt cascades with PKC activation in IGF-I, HNF1α, and CREB mRNA expression at the hepatic level. Carp hepatocytes were exposed to the PKC activator TPA (10 μM) for 12 h in the presence of (A) the MEK1/2 inhibitor PD98059 (10 μM) or ERK1/2 inhibitor SCH772984 (10 nM), or (B) the PI3K inhibitor Ly294002 (10 μM), Akt blocker API-2 (2 μM), or mTOR inactivator Rapamycin (20 nM). After that, total RNA was isolated from individual wells and subjected to real-time PCR with primers for IGF-I, HNF1α, and CREB, respectively.